For Dummies, 2011
ISBN: 9781118051108
Sprache: Englisch
Format: EPUB
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Title
Introduction
What This Book Does for You
Foolish Assumptions
How This Book Is Organized
Icons Used in This Book
Where to Go from Here
Part I : Getting Started in Bioinformatics
Chapter 1: Finding Out What Bioinformatics Can Do for You
What Is Bioinformatics?
Analyzing Protein Sequences
Analyzing DNA Sequences
Analyzing RNA Sequences
DNA Coding Regions: Pretending to Work with Protein Sequences
Working with Entire Genomes
Chapter 2: How Most People Use Bioinformatics
Becoming an Instant Expert with PubMed/Medline
Retrieving Protein Sequences
Retrieving DNA Sequences
Using BLAST to Compare My Protein Sequence to Other Protein Sequences
Making a Multiple Protein Sequence Alignment with ClustalW
Part II : A Survival Guide to Bioinformatics
Chapter 3: Using Nucleotide Sequence Databases
Reading into Genes and Genomes
Making Use (and Sense) of GenBank
Using a Gene-Centric Database
Working with Whole-Genome Databases
Exploring the Human Genome
Chapter 4: Using Protein and Specialized Sequence Databases
From Translated ORFs to Mature Proteins
Reading a Swiss-Prot Entry
Finding Out More about Your Protein
Chapter 5: Working with a Single DNA Sequence
Catching Errors Before It’s Too Late
Computing/Verifying a Restriction Map
Designing PCR Primers
Analyzing DNA Composition
Finding Protein-Coding Regions
Assembling Sequence Fragments
Beyond This Chapter
Chapter 6: Working with a Single Protein Sequence
Doing Biochemistry on a Computer
Doing Primary Structure Analysis
Predicting Post-Translational Modifications in Your Protein
Finding Known Domains in Your Protein
Discovering New Domains in Your Proteins
More Protein Analysis for Free over the Internet
Part III : Becoming a Pro in Sequence Analysis
Chapter 7: Similarity Searches on Sequence Databases
Understanding the Importance of Similarity
The Most Popular Data-Mining Tool Ever: BLAST
Controlling BLAST: Choosing the Right Parameters
Making BLAST Iterative with PSI-BLAST
Similarity Searches for Free over the Internet
Chapter 8: Comparing Two Sequences
Making Sure You Have the Right Sequences and the Right Methods
Making a Dot Plot
Making Local Alignments over the Internet
Making Global Alignments over the Internet
Using Lalign to Make a Global Alignment
Aligning Proteins and DNA
Free Pairwise Sequence Comparisons over the Internet
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